Holzhauer won his 28th straight game with an additional $130,222 in winnings on Monday. It was the second highest single-day sum ever recorded on the show. The professional sports gambler from Las Vegas also holds the all-time record, which is $131,127.
Before Holzhauer came along, the one-day record was held by Roger Craig on September 19, 2010, when he took home $77,000 in a single game.
That's $325,143 shy of Ken Jennings' $2,520,700, which he won in 74 episodes in 2004.
Since Holzhauer started competing on the game show in early April, he has shattered multiple records. He surpassed $1 million in the shortest time ever last month and most notably, he broke the show's record for single-day cash winnings on April 9th's episode.
What was even more exciting was that Holzhauer’s first record-breaking prize, $110,914, held a special numerical significance — it's his daughter birthday. She was born on November 9, 2014 (11/09/14).
“I said all along that I wanted to break Roger Craig’s one-game record and I did it,” Holzhauer said.
Fox News' Mariah Haas and the Associated Press contributed to this report.