Newly published research is rewriting the earliest chapter of the historical account of how the AIDS epidemic began in the United States.
The work, detailed in a study released Wednesday, discounts the long-held notion that a French-Canadian flight attendant, whose story was highlighted in the seminal book “And the Band Played On,” brought the virus to the United States.
“This individual was simply one of thousands infected before HIV/AIDS was recognized,” said Richard McKay, one of the authors and a professor in the department of the history and philosophy of science at the University of Cambridge, in Britain.
The study, which was published in the journal Nature, found that attention for years focused on the flight attendant, Gaetan Dugas, because of a typographical mistake. Through a labeling error on Dugas’s case file, Patient O (the letter) became Patient 0 (zero) — and the characterization that he was the source of the US outbreak stuck in the popular press.
The “O” was meant to indicate that Dugas was from “outside of California,” where his case came to light.
Despite the fact the investigators never suggested Dugas had been the source of the outbreak, a mythology evolved, in part through the book and film version of “And the Band Played On,” which was written by journalist Randy Shilts, who contracted HIV and died in 1994.
Some of the findings from the new study were earlier reported at a scientific conference in Boston in March.
The first official word of a new and terrifying disease that was infecting men who had sex with men came in a dispatch from the Centers for Disease Control and Prevention in 1981.
The virus that become known as HIV — human immunodeficiency virus — was later recognized to have evolved from a virus that infected primates in sub-Sarahan African. The jump from primates to people is believed to have happened in the early 1900s.
Along with others, Michael Worobey, a University of Arizona professor who led the new study, has been working for years to retrace the path the virus took as it emerged from its source to fan out across the globe.
Worobey and colleagues tested blood samples taken in 1978 and 1979 from gay men in New York City and San Francisco as part of studies looking at the spread of hepatitis B in these populations.
The scientists tested the samples — drawn before the existence of HIV was known — for evidence of HIV antibodies.
Even then, 6.6 percent of the New York samples and 3.7 percent of the San Francisco samples were positive for HIV infection, suggesting the virus had been spreading for a while in these communities.
The team wanted to study the genetic sequences of the viruses in the blood. Plotting them against the sequences of the other HIV viruses can help to generate a timeline for an outbreak because scientists know the rate at which the virus mutates.
But viral RNA degrades over time and initially Worobey and his team could not extract enough genetic material to generate full genetic sequences from the RNA in the blood samples. After developing a new technique they describe as “jackhammering,” they managed to come up with full sequences for eight patients — five from New York and three from San Francisco.
There is only one HIV virus sequence older than these in the viral genetic databases. The information that can be gleaned from this trove of sequence information suggests the virus arrived in the US around 1971 — before Dugas became a flight attendant and began his travels.
The evidence suggests the virus came to the United States from the Caribbean. The amount of differences among the sequences indicates the virus had been spreading for several years by the late 1970s when the blood samples were taken, the authors said.
Worobey and his coauthors studied the genetic sequence of the virus that infected Dugas and compared it to the stored blood samples.
“We found no evidence that Patient 0 was the first person infected by this lineage of HIV-1,” they wrote in the paper.
Worobey cautioned that efforts to try to trace the origins of an outbreak shouldn’t be used to target blame.
“No one should be blamed for the spread of a virus that no one even knew about,” he insisted.