The “7 Rings” singer dethroned Gomez, 26, on Monday, Billboard reported. As of Tuesday morning, Grande, 25, had 148,391,669 followers compared to Gomez’s 146,303,811.
Grande’s new title comes after she’s apparently gained more than 13 million new followers in the last four months, according to Billboard.
Previously, Gomez replaced Taylor Swift as the most popular Instagram user with more than 103 million followers back in December 2016.
While Grande is known for her presence on Instagram, using it to connect with her fans, Gomez recently took a break from the social media platform from roughly September to January.
On Sept. 23, Gomez posted a selfie on Instagram, announcing to fans that she planned to take another "social media break."
"Again. As much as I am grateful for the voice that social media gives each of us, I am equally grateful to be able to step back and live my life present to the moment I have been given," she captioned the post. "Kindness and encouragement only for a bit! Just remember - negative comments can hurt anybody's feelings. Obvi," she wrote at the time.
The following month, a source told Fox News that Gomez was reportedly seeking treatment at an East Coast facility to tackle "emotional issues head-on."
While both Grande and Gomez have an impressive amount of followers, neither compare to soccer star Cristiano Ronaldo, 34, who became the most-followed celebrity Instagram user in October with 144,580,697 followers at the time. Currently, he has 155,966,285.
Fox News' Jennifer Earl contributed to this report.