The couple posed with a portrait of the Duchess of Sussex in their video acceptance speech for the 2019 Brit Awards on Wednesday.
Beyonce, 37, and Jay-Z, 49, re-enacted their "APES—T" music video, which was filmed at the Louvre in Paris, in which they stood in front of the Mona Lisa.
Standing in front of a painting of Markle, 37, wearing a tiara, pearls and old-school royal regalia, Beyonce held her trophy and said, “Thank you so much to the Brit awards for this incredible honor. You guys have always been so supportive. Everything is love. Thank you.”
Jay-Z replied with a sly smile, "You're welcome."
They both then turn to gaze at the painting of the former "Suits" actress.
Beyonce later paid tribute to Markle on Instagram as well.
"Thank you to the Brits for the award for Best International Group. I won this award back in 2002 with my besties, Kelly and Michelle. How lucky am I to have been in a group with my other best friend, the GOAT Hova," Beyonce captioned a photo of the portrait moment with Jay-Z. "In honor of Black History Month, we bow down to one of our Melanated Monas. Congrats on your pregnancy! We wish you so much joy."