"Jennifer and Vince mutually agreed to end their relationship but continue to be good friends today," said representatives Stephen Huvane and John Pisani, according to People.com.
The representatives said the former co-stars decided to end their romance after Aniston visited Vaughn in London in October.
Aniston, 37, and Vaughn, 36, spent much of the last year and a half battling rumors about their relationship — first that it was starting, then that it was headed toward marriage, and most recently that it was sputtering.
The actors met while filming "The Break-Up" in 2005. Reports of a romance quickly swirled, but the two initially said they were simply friends.
They were spotted together in various places over the ensuing months but said little about their liaison. Still, by this summer, they were denying whispers of an engagement.
Within months, they were denying that they were breaking up. In October, Vaughn threatened to sue British and American tabloid newspapers that had reported that he was breaking it off with Aniston and had been seen kissing someone else.
Vaughn, now filming a holiday comedy called "Fred Claus," is known for his roles in such comic hits as "Wedding Crashers" and the 2004 film of "Starsky & Hutch." Aniston came to fame in the television comedy "Friends" and has gone on to star in a string of feature films.