Many of the media's questions involved the articles of impeachment being delivered to the Senate earlier in the day as well as the various claims made by Lev Parnas, an associate to Trump's personal attorney Rudy Giuliani.
"I don't know him. I have never had a conversation that I remember with him," Trump said about Parnas.
It was then that Acosta tried to squeeze in his own questions, asking about Parnas' claims that former National Security Adviser John Bolton and acting White House Chief of Staff Mick Mulvaney "knew" about efforts to withhold aid from Ukraine in exchange for announced investigations into the Bidens' dealings there.
"Quiet," the president told Acosta, and the reporter obliged.
Acosta, who has become known as an outspoken anti-Trump reporter for CNN in the Trump era, has clashed with the president several times at various news conferences since Trump took office.
The CNN reporter raised eyebrows last year with his book, "The Enemy of the People: A Dangerous Time to Tell the Truth in America," which many critics panned, arguing he sounded more like a "commentator" than a reporter with his repeated advocacy for journalists to push back strongly against the Trump administration.