NEW YORK – Scientists have grown human cells inside pig embryos, a very early step toward the goal of growing livers and other human organs in animals to transplant into people.
The cells made up just a tiny part of each embryo, and the embryos were grown for only a few weeks, researchers reported yesterday.
Such human-animal research has raised ethical concerns. The U.S. government suspended taxpayer funding of experiments in 2015. The new work, done in California and Spain, was paid for by private foundations.
Any growing of human organs in pigs is “far away,” said Juan Carlos Izpisua Belmonte of the Salk Institute in La Jolla, Calif., an author of the paper in the journal Cell.
He said the new research is “just a very early step toward the goal.”
Even before that is achieved, he said, putting human cells in animals could pay off for studies of how genetic diseases develop and for screening potential drugs.
Animals with cells from different species are called chimeras. Such mixing has been done before with mice and rats. Larger animals like pigs would be needed to make human-sized organs. That could help ease the shortage of human donors for transplants.
The Salk team is working on making humanized pancreases, hearts and livers in pigs. The animals would grow those organs in place of their own, and they’d be euthanized before the organ is removed.
Most of the organ cells would be human. By injecting pig embryos with stem cells from the person who will get the transplant, the problem of rejection should be minimized, said another Salk researcher, Jun Wu. Daniel Garry of the University of Minnesota, who is working on chimeras but didn’t participate in the new work, called the Cell paper “an exciting initial step for this entire field.”
Here’s what the new paper reports:
Scientists used human stem cells, which are capable of producing a wide variety of specialized cells. They injected pig embryos made in the lab with three to 10 of those cells apiece, and implanted the embryos into sows. At three to four weeks of development, 186 embryos were removed and examined.
Fewer than 1 in every 100,000 embryonic cells was human, which still comes to about a million human cells, Wu said. That contribution is lower than expected, he said, “but we were very happy to see we actually can see the human cells after four weeks of development.”
The cells generated the precursors of muscle, heart, pancreas, liver and spinal cord tissue in the embryos. The researchers said they plan to test ways to focus human cells on making specific tissues while avoiding any contribution to the brain, sperm or eggs.
That addresses ethical concerns that the approach could accidentally lead to pigs that gain some human qualities in their brains, or make human egg or sperm.
The government, meanwhile, has signaled that it may lift the federal funding ban soon. but impose extra oversight of any proposed work.