A federal investigation determined there was no threat aboard an American Eagle flight on which a flight attendant reported that two passengers were acting suspiciously, an FBI spokesman said early Saturday

The FBI searched the plane and interviewed the 27 passengers and three crew members aboard American Eagle Flight 4518 from Jackonsville, Fla., and determined "the observed activity was misperceived," FBI spokesman Newsom Summerlin in Charlotte said.

He would not comment on what the flight attendant thought she saw.

No one was arrested, he said.

Flight 4518 left Jackonsville, Fla., about 7:10 p.m. The report of suspicious activity occurred about 30 minutes before the plane landed at 8:40 p.m. in a remote area of the airport, Summerlin said.

Passengers were taken by bus from that location to a terminal about 9:45 p.m., the Transportation Security Administration said.