Vikings head coach Leslie Frazier announced the news on Tuesday.
"Antoine (Winfield) is going to be having surgery tomorrow on his clavicle. He fractured it. He's going to have surgery [Wednesday] so that's going to put him down for the remainder of the season," Frazier said.
Winfield, who has made the last three Pro Bowls, missed four straight games with a neck strain before returning Monday night and suffering the season- ending injury.