Hillis was limited in practice on Thursday but was expected to start before re-injuring his hamstring during practice on Friday and undergoing an MRI later that afternoon. He was officially ruled out Saturday.
The fourth-year back will miss his third straight game due to the injury. He last played in a 24-17 loss to the Raiders on October 16, and has 60 carries for 211 yards and two touchdowns this season.
Additionally, defensive lineman Auston English (knee) and cornerback Dimitri Patterson (knee) were both ruled out for Sunday.