The tournament announced the Australian's withdrawal Sunday morning. He was scheduled to play Albert Montanes of Spain on Day 1 at Roland Garros.
The 30-year-old Hewitt won the U.S. Open in 2001, and Wimbledon in 2002, and spent a total of 80 weeks at No. 1 in the ATP rankings.
He hasn't played on tour since March because of foot surgery.