The Southwestern Athletic Conference on game Pine Bluff football teams last Saturday.
Suspended were 25 UAPB players and 16 from Southern. The conference also assessed undisclosed fines against the universities.
Most of the suspensions will be for one game, but eight players will miss two for using "excessive force," the league said.
"What took place following Saturday's Southern/UAPB game has no place in college football," SWAC commissioner Duer Sharp said. "The conference is taking this action because of the nature and seriousness of the situation. Officials at both Southern University and the University of Arkansas at Pine Bluff have been very cooperative in addressing this issue with the conference office, and we thank them for their cooperation. These suspensions send a clear and definitive message that this type of behavior will not be tolerated."
The melee occurred after Southern scored a touchdown with no time remaining to pull within 22-21. UAPB's Jarvis Webb then blocked Matthew Hill's extra-point attempt, preserving the conference win for UAPB.
A shoving match broke out between the two teams shortly after the blocked kick and punches were thrown. Fans went on the field to join in the melee.
Southern players suspended for two games were LaMarkius Pettaway, Byron Williams and Jamie Payton.
Suspended for one game were Lee Doss, Jaleel Richardson, William Waddel, Terrell Alex, Levi Jackson, Marlon Smith, Kaelan Mayfield, Anthony Balancier, Franchot West, Kenneth Hill Jr., Corry Roy, LaQuinton Evans and Daniel Brown.
UAPB players suspended for two games were Dezmond Beverly, Stephen Jones, Chukwaso Oputa, Lyron McClenney and Devontaa Bryant
Suspended for one game were Cordara Frazier, Ryan Shaw, Mose Frazier, Joe Dalton, Kevin Rucker Jr., Justin Billings, Charles Williams, Kendrick Gray, Antonio Richmond, Torrence Shelton, Jr., Jherron Harris, Derrick Timber, Brandon Fulton, Devonne Tingle, K.J. Smith, Jamaal Philips, Colton Jones, Donnie Scott, Eric Thomas and Damien Lee.
Both teams have byes this weekend. Southern next plays Alcorn State Oct. 29 and visits Texas Southern Nov. 5, while Arkansas-Pine Bluff's next two games are against Grambling State Oct. 29 and Alabama State Nov. 5.