Updated

The latest on a deadly Greyhound bus accident (all times local):

11 a.m.

Greyhound says a bus that rolled on its side in Northern California and killed two passengers began its trip in Los Angeles.

Company spokeswoman Lanesha Gipson says the bus left at 11:30 p.m. Monday and crashed at 6:40 a.m. on northbound Highway 101 in San Jose, a major commuting thoroughfare.

Authorities say at least eight people were hospitalized, including one with major injuries.

Gipson says the bus driver began his shift in Los Angeles and that the company requires operators to rest nine hours between trips.

The accident is under investigation, and authorities say the driver will be asked to submit a blood sample to test for alcohol and drugs.

Gipson says the bus was carrying 20 passengers in addition to the driver.

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9:30 a.m.

The California Highway Patrol will ask the Greyhound bus driver involved in a crash that killed two women and injured several other people to submit a blood sample for alcohol and drug testing.

CHP officer Chris Miceli says the local district attorney has also been notified of the crash that snarled the rainy Tuesday morning commute in San Jose.

Miceli said that's a routine measure for bus accidents and that the cause of the wreck is under investigation.

Greyhound representatives didn't immediately return phone calls.

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8:20 a.m.

Authorities say a Greyhound bus rolled onto its side during a rainy morning commute in Northern California, killing two people and injuring 18 others.

The San Jose Fire Department reported that the bus flipped on its side while traveling north on Highway 101 in San Jose around 6:40 a.m. Tuesday. Authorities are investigating the cause of the accident. No other vehicles were involved.

The California Highway Patrol said 39 passengers and the driver were aboard the bus when it flipped onto the center divider.

The CHP said four people suffered moderate injuries, and 13 others suffered minor injuries. Four of the injured were transported to area hospitals. A young boy was also taken to the hospital as a precaution, the CHP said

The accident snarled a morning commute already slowed by rain.

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This story has been corrected to reflect that there were 20 passengers on the bus, not 39.