The suit was filed on behalf of the Jim Preminger Agency (search), which represented Peter Casey and David Lee, and the Kaplan Stahler Gumer Braun Agency, whose client was David Angell.
Angell and his wife were aboard one of the planes that crashed into the World Trade Center on Sept. 11, 2001.
The lawsuit alleges Paramount Pictures, which produced and distributed "Frasier," did not comply with the agreed upon agency package commission that is typically based on a percentage of revenues and profits generated by a series.
"Paramount has made other significant calculation errors ... that have resulted in an underpayment of additional amounts which are also now due and payable," according to the lawsuit.
The studio has made more than $1.5 billion in gross revenue from "Frasier," the suit claims.
Angell, Casey and Lee created, wrote and served as executive producers on the Emmy Award-winning comedy starring Kelsey Grammar.
A call left late Thursday for Paramount Pictures was not immediately returned.