Real Madrid will launch a fresh appeal after their expulsion from the Copa del Rey for fielding an ineligible player was upheld by the Spanish Football Federation.
The RFEF announced on Thursday that Madrid's disqualification would stand after Denis Cheryshev appeared - and scored the opening goal - in their 3-1 first-leg win over Cadiz on December 2 when the Russia international should have been serving a suspension.
Cheryshev received three yellow cards while playing for Villarreal in the competition last season.
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Madrid had appealed against the decision to award victory in the tie to Cadiz, insisting they had not been informed of the suspension.
A statement from the club said: "Real Madrid CF has received and analysed the resolution of the Appeals Committee, which once again verifies that the player Denis Cheryshev was not notified of his ban, the only way the aforementioned ban can take effect.
"The club has therefore submitted an appeal to the Administrative Court for Sport."
Madrid substituted Cheryshev at half-time in the match, with head coach Rafael Benitez saying the move had been as soon as the mistake was made to "show good faith".