Madonna dedicated her classic hit "Like a Virgin" to the pope at a sold-out concert in Rome on Saturday, Reuters reported.
"I dedicate this song to the pope, because I'm a child of God. All of you are also children of God," the 50-year old star told the 60,000 fans that flocked to the Italian stop of her "Sticky & Sweet" world tour.
Madonna, who comes from a devout Italian Catholic family, staged a mock-crucifixion at a 2006 concert in Rome to the backdrop of accusations from the Vatican of blasphemy.
The Vatican also condemned her controversial 1989 video for the song "Like a Prayer" that featured burning crosses, statues crying blood and Madonna seducing a black Jesus.
The Vatican later panned a show where she decked the stage out with religious imagery as one of the most "satanic shows in the history of humanity."