Vick suffered two broken ribs in last Sunday's loss to the Arizona Cardinals, forcing him to miss practice all week.
Maclin also sat out practice this week with shoulder and hamstring injuries. Both players were listed as questionable on Friday's injury report and were downgraded to out on Saturday.
Eagles offensive lineman King Dunlap (concussion) and cornerback Dominique Rodgers-Cromartie (high ankle sprain) were already ruled out.
Philadelphia is 3-6 this season and three games behind the first-place Giants in the NFC East.
Backup quarterback Vince Young took all the first-team reps in practice this week and will make his first start as an Eagle in place of Vick.