The relocation will clear the Rassvet port for the docking of another Soyuz vehicle, known as Soyuz MS-18.
Expedition 64 Roscosmos astronauts Commander Sergey Ryzhikov and Sergey Kud-Sverchkov, along with NASA Flight Engineer Kate Rubins, will undock from the Earth-facing port of the station's Rassvet module at around 12:38 p.m. ET.
They will dock again at the space-facing Poisk port at approximately 1:07 p.m. ET.
This expedition marks the 15th overall Soyuz port relocation and the first since August of 2019.
Rubins, Ryzhikov and Kud-Sverchkov are all slated to return to Earth aboard the Soyuz MS-17 on April 17.
Live coverage of the relocating will be aired on NASA TV, the NASA app and the agency's website.