Attendees at the February event may recall Vice President Mike Pence's speech being disrupted by a man dressed as the president and carrying a woman over his shoulders.
Moments later, the man many believed to be an anti-Trump protester was escorted out by authorities.
Several reporters described the commotion at the time.
As it turns out, that man was Cohen, who was filming a scene for "Borat Subsequent Moviefilm," the follow-up to the 2006 mockumentary "Borat! Cultural Learnings of America for Make Benefit Glorious Nation of Kazakhstan."
In the trailer, Cohen's iconic character Borat Sagdiyev is seen putting on a Trump costume and running around the convention with a woman, depicted as Borat's daughter, over his shoulders.
It then cuts to Pence's CPAC speech, where Cohen is seen crashing the room and shouting to the VP, "I've got a girl for you!"
The first "Borat" film earned an Oscar nomination for Best Adapted Screenplay and netted Cohen a Golden Globe for best actor in a musical or comedy film.
The sequel is set to be released Oct. 23 on Amazon Prime Video.