"There is no time to lose, Venezuela has no food to eat," the sign reads.
These women came from Anzoategui by bus. The trip took more than 10 hours because of the military controls in the highway.
People demanded a date is set this year for a referendum to recall President Nicolas Maduro.
Grimaldo Lopez has had this flag since 2002. It has been autographed by four political prisoners who remain behind bars: Former Mayors Leopoldo Lopez and Antonio Ledezma, and activists Yon Goicochea and Carlos Melo.
Public employees participated in the protest, despite government's threats they would be fired.
More than a thousand people concentrated in one of the starting points of the opposition rally before 9:00 am.
The Sept. 1st rally was meticulously planned by the opposition as a last-ditch effort to force authorities to call a referendum before the year ends – otherwise Chavismo could stay in power until 2019.