Federal investigators said Sunday that a Seattle duck boat that crashed into a charter bus Thursday did not have an axle repair that had been recommended for some of the amphibious vehicles two years ago, as a fifth international college student died from injuries suffered in the crash.

Ride the Ducks International, which refurbished the boat in 2005, issued a warning to its customers two years ago about potential axle failure and recommended a specific repair or increased monitoring, NTSB member Earl Weener said.

"This particular duck had not had the fix," he told a news conference.

The warning included specific instructions for inspecting the area where the shaft could fail, as well as instructions for the repair, which involved welding collars around the axle shaft, Weener said. It wasn't clear what prompted the warning or how the potential failure was discovered, or whether it applied to all duck boats or only those that the company had refurbished, he said. It wasn't clear how many of the 100 duck boats in service nationally may have had the repair, he said.

Witnesses described seeing the duck boat's left front tire lock up before it veered into the bus on the Aurora Bridge, and federal investigators announced Saturday that they found the duck boat's left front axle sheared off -- though they said it wasn't clear if the axle had broken before or after the collision.

Ride the Ducks International informed investigators late Saturday that it had issued the warning, he said. It's unclear if the company that owns the vehicle -- Ride the Ducks of Seattle -- was aware of the warning, Weener said.

"We're going to be following that," he said.

Washington state Gov. Jay Inslee and Seattle Mayor Ed Murray also announced Sunday that the state wants to suspend the company's operations pending the outcome of an investigation. Ride the Ducks had previously announced that it had suspended operations through Monday. The Washington Utilities and Transportation Commission will meet Monday to discuss its authority to shut down the company until that review is complete, Inslee said.

Meanwhile, Harborview Medical Center said late Sunday afternoon that a 20-year-old woman had passed away. Her name was not immediately released. She had previously been listed in critical condition.

The five who died in the crash were among about 45 students and staff from North Seattle College who were on the bus on the six-lane bridge with no median when the tourist-carrying duck boat swerved into it.

More than 50 people were taken to hospitals. At least 13 people remained at Seattle hospitals Sunday, with four in intensive care and one in critical condition.

The amphibious vehicle tours are offered around the world, including in Philadelphia; Austin, Texas; Miami; Fort Lauderdale, Fla.; and London. They feature former military landing craft repurposed as tour vehicles.

The vehicle involved in the Seattle crash was an Army surplus craft built in 1945. It was refurbished with a General Motors engine and chassis in 2005, Weener said.

Results of the federal probe are not expected for a year, he said. Investigators have interviewed 11 surviving passengers, including eight who were on the duck, as well as first responders. They expect to interview the drivers of the charter bus and the duck this week.

Weener described Ride the Ducks of Seattle as cooperative, and said the company had turned over training records and maintenance documents for the duck.

It's the first time the NTSB has looked into a land crash of the amphibious vehicles, which critics say are too dangerous for city streets. The federal agency has scrutinized duck tour vehicles several times when they've been in accidents on water.

The Associated Press contributed to this report.