For the second straight year, ABC led nominees for awards from an organization that monitors depictions of gays and lesbians in the media.
The network landed nine nominations from the Gay and Lesbian Alliance Against Defamation, while CBS garnered three nods, NBC two and Fox two.
ABC's nominated shows include "Brothers & Sisters," "Ugly Betty" and "Desperate Housewives." Among cable networks, Logo received five nominations, CNN four, Bravo three and ABC Family two.
Nominees for feature film in wide release include Julie Taymor's Beatles musical "Across the Universe," the fantasy "Stardust" and "The Jane Austen Book Club."
Special prizes will go to singers Rufus Wainwright and Janet Jackson, MTV Networks president of entertainment Brian Graden, "My So-Called Life" actor Wilson Cruz, activist David Mixner and "The L Word" creator Ilene Chaiken.
The 19th annual GLAAD Media Awards -- with 172 nominees in 40 categories -- are split into four ceremonies, held in Los Angeles, New York, San Francisco and southern Florida in March, April and May.
"This year's extraordinary nominees exemplify the important strides we have made as a community toward creating a truly inclusive society," GLAAD President Neil Giuliano said in a statement.
The organization noted that transgender issues were addressed in numerous news reports and documentaries as well as in the TV shows "All My Children," "Cold Case" and "Dirty Sexy Money."
The nominees were announced Sunday night during the Sundance Film Festival.