"This is a private matter and there will be no further comment at this time," CeCe Yorke said in a statement.
Locklear, 44, first gained fame in 1981 as sexy Sammy Jo Dean Carrington on the TV series "Dynasty."
In the 1990s, she took on the role of cold-hearted Amanda Woodward on TV's "Melrose Place" and played mayoral love interest Caitlin Moore on "Spin City."
Sambora, 45, is a founding member and lead guitarist for the rock band Bon Jovi. He has also released two solo albums.
Locklear and Sambora were married in 1994. Their daughter, Ava Elizabeth, is 8.
Locklear was previously married to Motley Crue drummer Tommy Lee.