The 13,880-square-foot house and 4.6-acre property is on the edge of Old Hickory Lake. Seven pieces of antique furniture, including their bed, are included in the sale.
"No one in the family was in a position to live there," said Cathy Sullivan, the estate's co-trustee. "That home has so many happy memories, for anyone to stay there it would not have seemed the same."
The house needs some maintenance work but is otherwise in "very good condition," said Johnny Cash's brother, Tommy Cash, who is the acting real estate agent.
Johnny Cash became a towering figure in American music with hits such as "Folsom Prison Blues," "I Walk the Line" and "A Boy Named Sue." Both Cash and his wife died in 2003.
Tommy Cash said that during his impromptu visits to the couple's home, he met everyone from President Carter to actor James Garner.
"If those walls could talk, it would be the biggest book in the world," he said.