Liverpool duo Jordon Ibe and Joe Gomez and Everton's Brendan Galloway are among 14 uncapped players to be included in the England Under-21 squad.
Manchester United forward James Wilson and Tottenham midfielder Delle Ali are also part of the uncapped group within the 23-man squad for the forthcoming matches against the United States and Norway.
The Young Lions, looking to bounce back from a disappointing European Championship campaign over the summer, face USA Under-23s next Thursday at Preston's Deepdale before taking on Norway Under-21s away the following Monday.
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Despite being eligible Manchester City's Raheem Sterling, Everton's John Stones and Manchester United's Luke Shaw were all left out, with them likely to be in Roy Hodgson's senior squad instead.
There are eight players who have been capped previously at Under-21 level, with Arsenal's Calum Chambers, Tottenham's Eric Dier and Southampton's Matt Targett on hand to help the new faces at the back.
James Ward-Prowse, Nathaniel Chalobah, Ruben Loftus-Cheek and Jake Forster-Caskey return to provide a wealth of know-how in midfield, while Norwich flyer Nathan Redmond also features again.
Wilson, Sunderland's Duncan Watmore and Wolves duo Kortney Hause and Dominic Iorfa make the step-up from last year's Under-20s, along with Arsenal's Chuba Akpom.
England Under-21s squad in full: Angus Gunn (Manchester City), Jordan Pickford (Preston North End - on loan from Sunderland), Christian Walton (Bury - on loan from Brighton), Calum Chambers (Arsenal), Eric Dier (Tottenham), Brendan Galloway (Everton), Joe Gomez (Liverpool), Kortney Hause, Dominic Iorfa (both Wolves), Jack Stephens (Middlesbrough - on loan from Southampton), Matt Targett (Southampton), Dele Alli (Tottenham), Nathaniel Chalobah (Chelsea), Jake Forster-Caskey (Brighton), Ruben Loftus-Cheek (Chelsea), James Ward-Prowse (Southampton), Chuba Akpom (Hull - on loan from Arsenal), Jordon Ibe (Liverpool), Solly March (Brighton), Nathan Redmond (Norwich), Duncan Watmore (Sunderland), James Wilson (Manchester United), Cauley Woodrow (Fulham).