Colts rookie offensive lineman Khaled Holmes was carted off the field with a right ankle injury during Tuesday afternoon's training camp practice.
Team officials did not have an update on the severity of the injury.
Backup linebacker Scott Lutrus left practice early with a knee injury and safety Sergio Brown left with a hamstring injury. Also missing was two-time Pro Bowl safety Antoine Bethea, who left camp to attend the birth of his daughter.
Free agent running back Ahmad Bradshaw, who is on the physically unable to perform list, said he expects to be ready to play in two to three weeks.
Indy did get one player back — linebacker Josh McNary, who spent the past two years fulfilling his service commitment to the Army.