Diana Taurasi netted a game-high 18 points while Tina Charles had 16 points and 14 rebounds as the United States rolled past the Czech Republic, 88-61, on Friday.
It was the first meeting between the teams since the gold medal game at the 2010 FIBA World Championship in Karlovy Vary, Czech Republic, which the USA won 89-69.
Maya Moore contributed 14 points and Angel McCoughtry finished with eight points and 11 boards for the United States, which improved to 4-0 in these Olympics.
"Each game we've had longer and longer stretches where we have been consistently good on offense. Defensively we're getting better," U.S. head coach Geno Auriemma said. "We've had longer and longer stretches where we've been very good. That's what you have to hope for at this point, just make sure we get better every game."
Michaela Zrustova paced the Czech Republic with 15 points. Eva Viteckova was the only other double-digit scorer with 12 in defeat.
The Czechs fell to 1-3 in Group A and will play Angola on Sunday. The U.S. will face China on Sunday.
The Czech Republic opened the game with a 10-0 run to take the early advantage, but USA answered with a 13-2 spurt to take a one-point lead just past the midpoint of the first quarter.
After a Seimone Augustus three-pointer put the U.S. on top, 22-17, a few minutes later, the Czech Republic closed out the first quarter with a 9-2 push to carry a 26-24 lead heading into the second.
It was the first time in the tournament that the Americans had trailed at the end of the first quarter.
"We were shaky at times in the first quarter," said Moore. "It wasn't what we wanted."
The Czech Republic would stretch its lead up to 30-26 with a Hana Horakova field goal 2 1/2 minutes into the second quarter, but the U.S. took over from there, closing out the half with a 22-8 run to take a 48-38 lead into the break.
The United States then dominated the second half, outscoring the Czech Republic by a mark of 22-9 in the third quarter to take a 70-47 lead into the fourth, where the U.S. held an 18-14 scoring advantage.