Ordonez was taken out of the Tigers' series-opening loss to the Texas Rangers in the fifth inning on Saturday, and it was later revealed that the 37-year-old had broken the same ankle that forced him to miss the second half of the 2010 season.
"We didn't have all the facts and details, but we've had some medical work done this morning," Tigers manager Jim Leyland told the team's website (Detroit.tigers.mlb.com).
"He will be done for the season."
The Tigers, who trail 1-0 in their best-of-seven American League Championship Series against the Rangers, are already short on outfielders.
Ordonez has played with the Tigers since 2005 and was hitting .385 during the playoffs.
Game Two of the series was postponed on Sunday because of rain in Texas and has been rescheduled for Monday.
(Writing by Jahmal Corner in Los Angeles; Editing by John O'Brien)