On Kansas City's opening drive of the game, Charles took a handoff out to the right side in an attempt to reach the first down marker on 3rd-and-4. He made it three yards before running out of room and being pushed out of bounds.
He tripped and fell to the ground and appeared to be favoring his left knee. He was eventually taken into the locker room on a cart.
Named to the Pro Bowl last season, the four-year veteran had 10 carries for 56 yards in last week's loss to Buffalo.