The Toronto third baseman, who leads the majors with 31 home runs, is listed as day to day.
Bautista was injured when he caught a spike in the dirt while sliding into third base in Toronto's 16-7 win over the Yankees on Thursday night at Rogers Centre. X-rays were negative and Bautista was re-evaluated Friday morning.
Jays manager John Farrell says Bautista's ankle was no worse than it was Thursday and that the All-Star is wearing a walking boot to help stabilize the injury.
Farrell said it's possible Bautista could return Sunday.
Edwin Encarnacion replaced Bautista at third base on Friday night.