Venus Williams lost to Daniela Hantuchova 6-2, 5-7, 6-2 in the quarterfinals Thursday, exiting Eastbourne a day after her sister was defeated but still pleased with her Wimbledon preparation in her return to tennis.
Both sisters are back after lengthy injury breaks. Venus was playing her first event in five months since retiring with hip and abdominal injuries during the Australian Open in January.
Venus said she heads to Wimbledon with plenty of confidence as she targets a sixth title at the All England Club. The tournament begins Monday.
"I played a lot of matches here, spent a lot of time on the court," Williams said. "It's not the best luck today, but I feel good about my preparation.
"I feel really positive about my matches here. I thought I played pretty good. I thought more than anything I was able to get a lot of great competition. This has been ideal."
Hantuchova had previously won only two sets in 10 previous meetings with Venus. But she has been in good form recently, reaching the Birmingham final last week.
She advances to a semifinal against fifth-seeded Petra Kvitova, who beat Agnieszka Radwanska 1-6, 6-2, 7-6 (2).
"I was really looking forward to the match today, and really pleased that I was able to win," Hantuchova said. "It's obviously nice to see (the Williams sisters) both back. I think they're great for the game."
Samantha Stosur defeated top-seed Vera Zvonareva 4-6, 7-6 (4), 6-4 on Thursday, the seventh straight win over her Russian opponent. Zvonareva needed three sets Wednesday to defeat Serena Williams, who was sidelined nearly a year because of foot surgeries and blood clots in her lungs.
Zvonareva led 4-2 in the second set against Stosur, but lost the tiebreaker. Stosur won the first four games of the deciding set en route to victory.
Stosur will meet Marion Bartoli in the semifinals on Friday. Bartoli advanced when 2010 runnerup Victoria Azarenka retired with a right groin injury while trailing 6-2, 2-0.
Hantuchova dominated the listless Williams in the first set, serving well, playing aggressively and showing confidence in going for the lines.
The former No. 5-ranked Slovak broke in the third game after Williams double-faulted and then made a backhand error. She broke again to lead 5-2 with a superbly struck backhand pass down the line.
Williams offered a brief challenge as Hantuchova served for the set but failed to convert three break points. Hantuchova closed out the set with a crosscourt winner.
"I definitely wish I could have came out harder," Williams said. "I think she was a little bit tentative in the beginning, and then to lose serve wasn't ideal, but I definitely thought I was gonna break back. That didn't work out the way I wanted to."
Hantuchova held a break point in the second set to lead 5-3. But she hit a ball down the line that landed wide, and Williams went on to break for 4-4 when her return clipped the net and fell in her favor.
Williams broke at love for the set when Hantuchova netted a weak backhand.
With both players battling a strong wind, Hantuchova regained the upper hand in the final set, breaking for 1-0. Williams made a brief revival, leveling at 2-2 before Hantuchova swept the next four games.
Williams, who mishit a serve that flew out of the stadium, acknowledged that the wind had been a factor.
"Today was really tough," she said. "It was hard to capitalize on the serve. Right up until the last point, I took my racquet back and the wind blew it forward. I was like, 'Oh, no.'
"But I give her credit to hanging there."
Despite competing well this week, Williams said she would have preferred more time before returning to the tour — but that Wimbledon was too important to pass up.
"This is Wimbledon, after all. So I didn't want to miss it," she said. "But regardless, I think I'm playing pretty well."
In the men's event, third-seeded Serb Janko Tipsarevic defeated Bulgaria's Grigor Dimitrov 6-3, 7-6 (2), Igor Kunitsyn of Russia beat Julien Benneteau of France 6-3, 6-2, Italy's Andreas Seppi outlasted Olivier Rochus of Belgium 6-2, 6-7 (5), 6-3 and Japan's Kei Nishikori overcame Czech Radek Stepanek 6-2, 6-3.