The 45-year-old Hopkins (50-5-1, 32 KOs) is a former middleweight and light heavyweight champion. He defeated Enrique Ornelas by unanimous decision in a light heavyweight bout in Philadelphia in December.
The 41-year-old Jones (54-6, 40 KOs) is coming off a loss to Australian Danny Green in the first round, also in December.
On May 22, 1993, in Washington, D.C., Hopkins lost a unanimous decision to Jones for the vacant IBF middleweight crown.
The rematch is scheduled for April 3 at the Mandalay Bay Events Center and will be broadcast on pay-per-view.