Orange County Health Department spokesman Dain Weister on Monday wouldn't say how old the woman is because of patient confidentiality. But he says she got sick shortly after moving to the Orlando area last month.
The woman was treated by a doctor who gave her antibiotics. None of her close contacts have gotten sick.
There has been at least one other confirmed case in Florida of the disease caused by a bacterial infection of the intestine. That Collier County case also has been linked to an outbreak in Haiti.
Doctors are trying to determine whether an American Airlines passenger who became ill last Friday on a flight from the Dominican Republic to Miami had cholera.