Phil Robertson included remarks in a sermon in his church West Monroe, Louisiana that harken back to the "Duck Dynasty" star's past anti-gay remarks.
Robertson included homosexuals with other groups, including thieves, drunks and adulterers, as hell-bound sinners in a sermon he gave on Easter that was recorded and put up on the church's website.
“Neither the sexually immoral, nor the idolators, nor adulterers, nor male prostitutes, nor homosexual offenders, nor thieves, nor greedy, nor drunkards, nor slanderers, nor swindlers will inherit the kingdom of God,” Robertson said, paraphrasing a verse from Corinthians in the Bible.
Robertson's rep tells FOX411 that "he was in a church quoting the Bible," but would offer no further comment.
A&E has declined to comment on new video.
Robertson is the bearded patriarch of a clan that manufactures duck calls and became reality-TV stars. In December he set off a firestorm after GQ magazine quoted him linking homosexual behavior to bestiality. He also made racist statements.
A&E suspended him for nine days. Robertson issued an apology.
The video was posted Thursday by RadarOnline. It was posted to YouTube by the Whites Ferry Road Church on April 21.
"Duck Dynasty" is scheduled to begin its sixth season on June 11.
The Associated Press contributed to this report.