A show spokesman said Wednesday representatives of the NFL star called late Tuesday to cancel his appearance for personal reasons. The daytime talk show host's interview with Vick was set to air Thursday, Feb. 24.
The Philadelphia Eagles organization apparently voiced objections to Vick's planned appearance, causing him to cancel, according to MyFoxPhilly.com.
Vick issued a statement saying: "After careful consideration, I will need to postpone the taping of the Oprah Winfrey interview scheduled for February 22. I admire and respect Oprah and hope to be able to participate in an interview in the future."
The interview was to cover Vick's time in prison, his work with the Humane Society and his return to the NFL.
Vick missed two seasons and served 18 months in federal prison for a dogfighting operation based on his property in Surry County, Va.
He was reinstated to the NFL for the 2009 season and has been doing public service work, including with the Humane Society.
The Associated Press contributed to this report.